Leica FCS2

Cross-correlation detection and analysis system - extension of Leica TCS SP2

functional principle of FCS

Overview

Fluorescence Correlation Spectroscopy -
Analytical Extension of Leica TCS SP2 AOBS Confocal System

FCS (Fluorescence Correlation Spectroscopy) is a method to measure concentration and diffusion rates quantitatively down to the single molecule level. The data can be used to analyze molecule interaction and transport processes within living cells. It allows to evaluate the dynamics of cellular systems and structures.

The sensitive cross-correlation detection and analysis system is coupled to the external port of the Spectral Confocal Microscope, assuring perfect and alignment-free registration of molecules moving through the FCS measurement spot in the confocal image, a precon-
dition for meaningful results.

Key Features

  • Dual channel cross-correlation optics and FCS detection system on Spectral
  • Confocal Leica TCS SP2 AOBS
  • Perfect, pixel precise positioning accuracy of FCS measurement spot due to single scanner and optics for both systems
  • No additional adjustment necessary for FCS
  • 30-50% more light detected due to AOBS (Acousto-Optical Beam Splitter)
  • FCS sensitivity improved by 70-125%
  • High flexibility in choice of dyes and fluorescent proteins
  • Full Spectral Confocal with four fluorescence detector channels for regular imaging applications
  • Retrofit of Leica TCS SP2 AOBS systems
  • Data analysis on independent workstation keeps the Confocal free
  • Powerful, highly flexible data analysis tool
  • Raw data, auto and cross correlation functions, photon counting histogram available
  • Various model functions for data fitting, user defined functions