Internal Fast Filter Wheel IFW
Change in milliseconds between different fluorescence excitations
Fluorescence Center
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With the new internal filterwheel it is possible to excite fluorochromes in the range of 20-30 ms and switch very fast between three colours and absolutely vibration free.
Using multiple fluorescence labels within the cell is now commonplace for co-localizing events. In the past, this often required expensive external devices like fast-switching monochrometers to switch excitation wavelengths for each probe species. Now, the fast IFW in combination with a multi-bandpass emission filter cube allows nearly all GFP variants to be excited within 50 milliseconds.
Using multiple fluorescence labels within the cell is now commonplace for co-localizing events. In the past, this often required expensive external devices like fast-switching monochrometers to switch excitation wavelengths for each probe species. Now, the fast IFW in combination with a multi-bandpass emission filter cube allows nearly all GFP variants to be excited within 50 milliseconds.
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An example: the BGR filter cube with three excitation ranges 405 to 435, 487 to 502 and 560 to 580. The internal fast filterwheel consists of a short pass 460 filter, a bandpass 460 to 515 and a long pass 520 and higher. The filters on the wheel preselect a range out of the filtercube.
Furthermore the Leica FIM (Fluorescence Intensity Manager), 2 fast shutters, a motorized Excitation Manager are on a disk with a diameter of 49.5 mm.
Furthermore the Leica FIM (Fluorescence Intensity Manager), 2 fast shutters, a motorized Excitation Manager are on a disk with a diameter of 49.5 mm.
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